Translucent K+ free medium, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium and w/o Folic acid and w/o Riboflavin

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Description

Translucent K+ free medium, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium and w/o Folic acid and w/o RiboflavinIn Translucent K free medium ammonium phosphate (0. 92 g l) substitutes for potassium phosphate to reduce the amount of potassium to a minimum. Both Folic acid and Riboflavin are omitted from the medium to make it suitable for fluorescence measurements with yeast cultures. Suspend 1. 75 gram powdered medium in 1 litre distilled water Store dry at room temperature

Terrific Broth medium is used with glycerol as a carbon source

01 Suspend 16

MOPS is frequently used as a buffering agent in biology and biochemistry

Peptone 10 Beef Extract 10 NaCl 5 Agar 12 Final pH 7

2 at 25°C Suspend 20

LB-Agar Low Salt

8 L-Aspartic Acid 53

15 grams of powdered medium in 1 litre distilled water

2 KH2PO4 1

0025 Potassium Dihydrogen phosphate 0

Due to the high levels of peptides

33 Assay ≥99%