FM Minimal medium w/o Cysteine and w/o Methionine

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Description

FM Minimal medium w/o Cysteine and w/o MethionineThis media is based on well defined formulations of mineral salts, vitamins and amino acids. Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH. FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5. SIH medium is a newly developed modification of FM with regards to

2 at 25°C Suspend 75 gram powdered medium in 1 litre distilled water

Glutamic Acid

Keep container tightly closed and protect from light

Vitamins and Agar without Amino Acids

developed by Hanh

minus Cysteine

minus Methionine

Chloramphenicol is a bacteriostatic antibiotic agent against gram-negative and gram-positive bacteria

carbohydrates and water soluble vitamins of B group

CAS 7240-90-6

Adjust to pH 6

Due to the high levels of peptides

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