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Vegetal LoFlo MediumLoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long term culturing. Typically, cells are grown only overnight in LoFlo, and transferred to fresh Lo Flo the following morning prior to imaging, so that LoFlo growth limited to

3% Appearance White Crystalline Powder Store dry at room temperature

preventing the binding of aminoacyl transfer RNA and inhibiting protein synthesis and hence cell growth

12 pH (100 g/l) 5

001/ml RNase and DNase Removed Store at 4°C

Character Fine white powder Solubility Freely soluble in water Specific Optical Rotation 104 ~ 121 Water <6% Absorbance 570 nm <0

51 CoCl2 0

As a result yeasts are not only limited in Phosphate

05 Total 47

YPD medium (YEPD) is a complex medium for routine growth

Unlike glucose

Dextrose 30 Casamino acids 2 Agar 17 L-Histidine 0

HL5 supplemented with vitamins and micro-elements as present in FM and SIH Media