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LBM-Broth MillerLBM Broth High Salt In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells. Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption. Formula g l Tryptone 10 Yeast extract 5 NaCl 10 MgSO4. anhydrous 0. 98 Final pH 7. 0 0. 2 at 25C Suspend 26 gram powdered medium in 1 litre distilled water Store dry at room temperature

5% solution 100°C) 6

Suspend 35

Tryptone and Yeast extract do not contain enough Mg2 for optimal adsorption

1 gram powdered medium in 1 litre distilled water

Methionine and Tryptophan concentrations has been reduced to 10% of their original concentration as present in Yeast Nitrogen Base

coli cells

Agar has been omitted from the original formulation

0 M 60

galactose and raffinose

maximum water solubility and minimum solubility in all other solvents

Colour Light cream Mesh Size (ASTM / Retained on Mesh 80): >50 on mesh 80 >90 on mesh 200 Gel Strength (Nikkan method) 700 - 1000 g/cm2 Moisture <15% pH (1

CAS 3483-12-3

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